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In most cases (and in this case) yes. The only time you can't is when an upgrade has a minimum version requirement (which in that case, you'd need to upgrade to that version first, then upgrade the the lastest).Can I just upload and install 2.5.4
There is none. If you try and upgrade and there is one, the installer will throw an error message that states there is a minimum version requirement (and it will specify what that version is).
You can upgrade from SC 2.5.0 to SC 2.5.4 without having to run all the middle versions.